University of Miami Biology Department House Rabbit Society of Miami If your bunny has an infection of any kind--from an upper respiratory infectionto a jaw abscess to a urinary tract infection --it's critical to know which antibiotics will be effective against the particular pathogen i. This means that 1 the species and strain of bacteria or other pathogen must be identified and 2 the drugs most effective at inhibiting their growth must be determined. The only reliable way this can be done is a culture and sensitivity test. In modern laboratories, bacteria are usually identified by characterization of the genome:
Introduction It is not enough to just identify your organism. You also need to know what antimicrobial agents your organism is susceptible to. There are several methods to determine this. This is done by adding two-fold dilutions of the antimicrobial agent directly to an agar pour, a broth tube, or a micro-broth panel.
The lowest level that inhibits the visible growth of the organism is considered the Minimum Inhibitory Concentration MIC. The agar pour method is considered the reference test procedure in Europe. The broth dilution method is more widely accepted in North America.
The E test AB Biodisk is a plastic strip with a gradient concentration of antimicrobial agents impregnated in it.
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The strip is placed directly on the surface of an inoculated plate. The MIC is read from the strip where the growth inhibition intercepts the disk. These strips are relatively expensive. Many physicians however do not need no know the exact MIC, but just which antibiotics the pathogen is susceptible, intermediate, or resistant to.
The Kirby-Bauer agar diffusion method is well documented and is the standardized method for determining antimicrobial susceptibility. White filter paper disks 6 mm in diameter are impregnated with known amounts of antimicrobial agents.
Each disk is coded with the name and concentration of the agent. The drug diffuses through the agar. The plates are incubated for hours.
The agar may be supplemented with blood or you may use blood agar for fastidious organisms. The diameter of the visible zone of inhibition is measured and compared to reference values. There should be sufficient bacteria to form a visible lawn of growth where it is not inhibited by the drug.
The Microbiology Lab at Apollo Diagnostics provides techniques like microscopy, antimicrobial susceptibility tests etc. to treat infectious diseases. ANTIBIOTIC SENSITIVITY DISCS Vetlab Supply ORDER FORM SW 98 Court [email protected] srmvision.com 50/Cartridge Palmetto Bay FL Person Placing Order Email Date.
An antibiogram is an overall profile of antimicrobial susceptibility testing results of a specific microorganism to a compiled mainly by microbiology laboratory technologists, but may be a collaborative effort involving the lab, pharmacy, MDH Fact Sheet Author: MDH.
This section of the laboratory performs antimicrobial susceptibility testing (AST) and microbial identification (ID) for the whole laboratory. Susceptibility testing assists the clinician in the choice of antimicrobial agents for therapeutic or prophylactic use in the .